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Yeasen Biotechnology tunel staining kit
Tunel Staining Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tunel+staining+kit/kit+staining+tunel/pm42264139-138-6-9
Average 86 stars, based on 1 article reviews
tunel staining kit - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

TUNEL Assay:

Article Title: HDAC2-mediated H3K27ac Governs ZFP42 transcription and autophagy in granulosa cells of pigs.
Article Snippet: Aims: Histone deacetylation regulates mammalian follicular development via ovarian granulosa cells (GCs) autophagy, but the underlying mechanisms remain largely unknown.. This study aimed to elucidate the epigenetic role of HDAC2 in GC autophagy and follicular development through ZFP42.. Materials and methods: HDAC2 knockdown combined with autophagy detection were performed to identify the effect of HDAC2 on autophagy and ZFP42 in GCs.

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

Article Title: Investigation of the impact of resveratrol on sublethal-dose ALA-PDT in glioblastoma cells.
Article Snippet: .. The following reagents and chemicals were used in this study: Res (501360, Sigma, MO, USA); ALA (Shanghai Fudan-Zhangjiang Bio-Pharmaceutical Co, Ltd, China); CCK-8 reagent (40203ES), calcein-AM/Propidium Iodide (PI) staining kit (40747ES), TUNEL staining kit(E0307ES) (Yeasen Biotechnology Co., Ltd., Shanghai, China); RIPA lysis buffer (Epizyme Biotech, Shanghai, China). .. The 1 3 primary antibodies of p-ERK (AF5304), p-S6K (AF3228), p-S6 (AF3354), S6 (AF6354), p62 (AF5384), and GAPDH (AF7021) (Affinity Biosciences, Jiangsu, China), the primary antibodies of S6K (9202 S) and LC3B (3868T) (Cell Signaling Technology, Boston, USA).

Article Title: USP51/GRP78/ABCB1 axis confers chemoresistance through decreasing doxorubicin accumulation in triple-negative breast cancer cells
Article Snippet: .. Cell apoptosis was detected using a TUNEL staining kit (Yeasen). .. Three random microscopic fields per slide were examined under a BX53 microscope (Olympus, Tokyo, Japan).

Article Title: Glycine attenuates high fructose-induced liver injury in adolescent mice by modulating lipid metabolism and gut microbiota.
Article Snippet: Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content.. The journal’s standard Terms & Conditions and the Ethical guidelines still apply.. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.

Article Title: NLRP3 inflammasome constrains liver regeneration through impairing MerTK-mediated macrophage efferocytosis
Article Snippet: Subsequently, the primary antibody including anti-Ki67 (1:300; Affinity, no. AF0198) and anti-BrdU (1:200; ABclonal, no. A1482) were incubated overnight at 4°C, followed by incubation with the corresponding fluorescein-conjugated secondary antibodies. .. For TUNEL staining, the frozen sections were subjected to TUNEL staining kit (Yeasen), following the manufacturer’s instructions. .. 4′,6-Diamidino-2-phenylindole (DAPI; Servicebio) was used to stain cell nuclei.

Article Title: Exosomal CircRNA-MANBA Mediates Hepatocellular Carcinoma Sorafenib Resistance via miR-1290/CD109/p-STAT3 Axis
Article Snippet: After 48 hours of exposure to sorafenib, the medium was supplemented with CCK-8 reagent (Beyotime) and the plate was read at an optical density (OD) of 450 nm. .. Apoptosis assays and TUNEL experiments The apoptosis of HCC cells was evaluated with a FITC-conjugated Annexin V and TUNEL staining kit (Yeasen, Shanghai, China) following the instructions provided by the manufacturer. .. The detection of apoptotic cells was carried out through a BD Biosciences © FACS Aria II ow cytometer (Le Pont de Claix, France) and a Carl Zeiss© LSM510 confocal laser scanning microscope (Marly-le-Roi, France).

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways
Article Snippet: The results were analyzed under a microscope from Leica (Leica, Germany). .. Apoptosis in tumor tissues was assessed using a TUNEL staining kit (Yeasen, China). .. Three randomly selected microscopic fields per slide were examined under a microscope (Leica, Germany).

Staining:

Article Title: HDAC2-mediated H3K27ac Governs ZFP42 transcription and autophagy in granulosa cells of pigs.
Article Snippet: Aims: Histone deacetylation regulates mammalian follicular development via ovarian granulosa cells (GCs) autophagy, but the underlying mechanisms remain largely unknown.. This study aimed to elucidate the epigenetic role of HDAC2 in GC autophagy and follicular development through ZFP42.. Materials and methods: HDAC2 knockdown combined with autophagy detection were performed to identify the effect of HDAC2 on autophagy and ZFP42 in GCs.

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

Article Title: Investigation of the impact of resveratrol on sublethal-dose ALA-PDT in glioblastoma cells.
Article Snippet: .. The following reagents and chemicals were used in this study: Res (501360, Sigma, MO, USA); ALA (Shanghai Fudan-Zhangjiang Bio-Pharmaceutical Co, Ltd, China); CCK-8 reagent (40203ES), calcein-AM/Propidium Iodide (PI) staining kit (40747ES), TUNEL staining kit(E0307ES) (Yeasen Biotechnology Co., Ltd., Shanghai, China); RIPA lysis buffer (Epizyme Biotech, Shanghai, China). .. The 1 3 primary antibodies of p-ERK (AF5304), p-S6K (AF3228), p-S6 (AF3354), S6 (AF6354), p62 (AF5384), and GAPDH (AF7021) (Affinity Biosciences, Jiangsu, China), the primary antibodies of S6K (9202 S) and LC3B (3868T) (Cell Signaling Technology, Boston, USA).

Article Title: USP51/GRP78/ABCB1 axis confers chemoresistance through decreasing doxorubicin accumulation in triple-negative breast cancer cells
Article Snippet: .. Cell apoptosis was detected using a TUNEL staining kit (Yeasen). .. Three random microscopic fields per slide were examined under a BX53 microscope (Olympus, Tokyo, Japan).

Article Title: Glycine attenuates high fructose-induced liver injury in adolescent mice by modulating lipid metabolism and gut microbiota.
Article Snippet: Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content.. The journal’s standard Terms & Conditions and the Ethical guidelines still apply.. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.

Article Title: NLRP3 inflammasome constrains liver regeneration through impairing MerTK-mediated macrophage efferocytosis
Article Snippet: Subsequently, the primary antibody including anti-Ki67 (1:300; Affinity, no. AF0198) and anti-BrdU (1:200; ABclonal, no. A1482) were incubated overnight at 4°C, followed by incubation with the corresponding fluorescein-conjugated secondary antibodies. .. For TUNEL staining, the frozen sections were subjected to TUNEL staining kit (Yeasen), following the manufacturer’s instructions. .. 4′,6-Diamidino-2-phenylindole (DAPI; Servicebio) was used to stain cell nuclei.

Article Title: Exosomal CircRNA-MANBA Mediates Hepatocellular Carcinoma Sorafenib Resistance via miR-1290/CD109/p-STAT3 Axis
Article Snippet: After 48 hours of exposure to sorafenib, the medium was supplemented with CCK-8 reagent (Beyotime) and the plate was read at an optical density (OD) of 450 nm. .. Apoptosis assays and TUNEL experiments The apoptosis of HCC cells was evaluated with a FITC-conjugated Annexin V and TUNEL staining kit (Yeasen, Shanghai, China) following the instructions provided by the manufacturer. .. The detection of apoptotic cells was carried out through a BD Biosciences © FACS Aria II ow cytometer (Le Pont de Claix, France) and a Carl Zeiss© LSM510 confocal laser scanning microscope (Marly-le-Roi, France).

Article Title: Lasalocid inhibits melanoma by down-regulating FOXM1 through PI3K/AKT and JNK/P38 MAPK pathways
Article Snippet: The results were analyzed under a microscope from Leica (Leica, Germany). .. Apoptosis in tumor tissues was assessed using a TUNEL staining kit (Yeasen, China). .. Three randomly selected microscopic fields per slide were examined under a microscope (Leica, Germany).

Fluorescence:

Article Title: HDAC2-mediated H3K27ac Governs ZFP42 transcription and autophagy in granulosa cells of pigs.
Article Snippet: Aims: Histone deacetylation regulates mammalian follicular development via ovarian granulosa cells (GCs) autophagy, but the underlying mechanisms remain largely unknown.. This study aimed to elucidate the epigenetic role of HDAC2 in GC autophagy and follicular development through ZFP42.. Materials and methods: HDAC2 knockdown combined with autophagy detection were performed to identify the effect of HDAC2 on autophagy and ZFP42 in GCs.

Magnetic Beads:

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

Protease Inhibitor:

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

Bicinchoninic Acid Protein Assay:

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

SYBR Green Assay:

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

Polymerase Chain Reaction:

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

Reverse Transcription:

Article Title: Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Article Snippet: LI-COR technology (NE, USA) supplied us with IRDye 800CW labeled goat anti-rabbit IgG and IRDye 680CW labeled goat anti-mouse IgG. .. We also used a range of other reagents from various suppliers for different purposes in our experiments, including the NOL12 fusion protein (Ag7738) from Proteintech Group (IL, USA); streptavidin–biotin-peroxidase complex, Hoechst 33258, DAB, His-tag affinity magnetic beads, PMSF, and a protease inhibitor cocktail from Sigma-Aldrich (MO, USA); RPMI 1640 medium, Lipofectamine 2000 TM , Pierce BCA Protein Assay Kit, and TRIzol reagent from Thermo Fisher Scientific (MA, USA); Protein A + G beads from Beyotime (Shanghai, China); ATR inhibitor VE822, ATM inhibitor ku55933, Caspase inhibitor Z-VAD-FMK, and calpain inhibitor calpeptin from MedChemExpress (NJ, USA); Alamar Blue kit, TUNEL staining kit, and SYBR Green PCR Mix Kit from Yeasen Biotech (Shanghai, China); Annexin V/PI Apoptosis Detection Kit from KeyGEN (Jiangsu, China); Taqman Universal PCR mix from Tiangen Biotech (Beijing, Chian); GoScriptTM reverse transcription kit from Promega (WI, USA). .. Sprague Dawley (SD) rats, including non-pregnant rats aged 6–8 weeks, pregnant rats on day 13.5 of pregnancy, and newborn SD rats at 1 day postpartum, were obtained from the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China).

CCK-8 Assay:

Article Title: Investigation of the impact of resveratrol on sublethal-dose ALA-PDT in glioblastoma cells.
Article Snippet: .. The following reagents and chemicals were used in this study: Res (501360, Sigma, MO, USA); ALA (Shanghai Fudan-Zhangjiang Bio-Pharmaceutical Co, Ltd, China); CCK-8 reagent (40203ES), calcein-AM/Propidium Iodide (PI) staining kit (40747ES), TUNEL staining kit(E0307ES) (Yeasen Biotechnology Co., Ltd., Shanghai, China); RIPA lysis buffer (Epizyme Biotech, Shanghai, China). .. The 1 3 primary antibodies of p-ERK (AF5304), p-S6K (AF3228), p-S6 (AF3354), S6 (AF6354), p62 (AF5384), and GAPDH (AF7021) (Affinity Biosciences, Jiangsu, China), the primary antibodies of S6K (9202 S) and LC3B (3868T) (Cell Signaling Technology, Boston, USA).

Lysis:

Article Title: Investigation of the impact of resveratrol on sublethal-dose ALA-PDT in glioblastoma cells.
Article Snippet: .. The following reagents and chemicals were used in this study: Res (501360, Sigma, MO, USA); ALA (Shanghai Fudan-Zhangjiang Bio-Pharmaceutical Co, Ltd, China); CCK-8 reagent (40203ES), calcein-AM/Propidium Iodide (PI) staining kit (40747ES), TUNEL staining kit(E0307ES) (Yeasen Biotechnology Co., Ltd., Shanghai, China); RIPA lysis buffer (Epizyme Biotech, Shanghai, China). .. The 1 3 primary antibodies of p-ERK (AF5304), p-S6K (AF3228), p-S6 (AF3354), S6 (AF6354), p62 (AF5384), and GAPDH (AF7021) (Affinity Biosciences, Jiangsu, China), the primary antibodies of S6K (9202 S) and LC3B (3868T) (Cell Signaling Technology, Boston, USA).



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